introduction to bacteriology:
brief overview to media agar types:
SHEEP BLOOD AGAR or TRYPTICASE SOY AGAR WITH 5% SHEEP BLOOD (SBA, BAP or TSA):
CHOCOLATE AGAR (CHOC):
The image above is a lactose-fermenting strain of E. coli growing on the left (normal fecal flora), and a non-lactose fermenting strain of E. coli growing on the right. Further testing must be done to determine whether or not this strain is the pathogenic enterohemorrhagic E. coli 0157:H7, or if it is a normal strain part of the fecal flora.
MACCONKEY AGAR (MAC):
MACCONKEY AGAR WITH SORBITOL (SMAC):
HEKTOEN AGAR (HEK):
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TCBS AGAR
COLUMBIA HORSE BLOOD AGAR (CDC):
CDC Anaerobic Blood Agar (referred to as CDC or CABA) is an enriched medium for the growth and partial identification of obligate anaerobes. It also supports good growth of aerobic, facultative anaerobic and microaerophilic organisms found in clinical materials if incubated under the appropriate conditions. CDC Anaerobic Blood Agar is a tryptic soy agar base supplemented with vitamin K1 and hemin to facilitate the recovery of more fastidious organisms, such as Prevotella, Porphyromonas, and the Bacteroides fragilis group, and should facilitate pigment production of Prevotella melaninogenica. Sheep blood is added for the observation of hemolytic reactions as seen by the double zone beta – hemoylsis of Clostridium perfringens. This medium is prepared, dispensed and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
CDC Anaerobic Blood Agar (referred to as CDC or CABA) is an enriched medium for the growth and partial identification of obligate anaerobes. It also supports good growth of aerobic, facultative anaerobic and microaerophilic organisms found in clinical materials if incubated under the appropriate conditions. CDC Anaerobic Blood Agar is a tryptic soy agar base supplemented with vitamin K1 and hemin to facilitate the recovery of more fastidious organisms, such as Prevotella, Porphyromonas, and the Bacteroides fragilis group, and should facilitate pigment production of Prevotella melaninogenica. Sheep blood is added for the observation of hemolytic reactions as seen by the double zone beta – hemoylsis of Clostridium perfringens. This medium is prepared, dispensed and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
CNA AGAR: For recovery of Gram-positive organisms
CDC KV LAKED AGAR: For recovery of Gram-negative anaerobes
FASTIDIOUS ANAEROBE AGAR:
NUTRIENT AGAR:
THIOGLYCOLLATE BROTH:
Thioglycollate broth, or THIO, is used as a medium to test growth of microbes. It is to be stored at room temperature, in the dark so that there is a decreased absorption of oxygen by the medium.
Thioglycollate broth, or THIO, is used as a medium to test growth of microbes. It is to be stored at room temperature, in the dark so that there is a decreased absorption of oxygen by the medium.
CAMPYLOBACTER (CVA) AGAR:
BILE ESCULIN TEST:
SABOURAUD DEXTROSE AGAR
MUELLER-HINTON AGAR
POTATO DEXTROSE AGAR
STREPTOCOCCUS AGAR (SSA)
CORNMEAL AGAR
LOWENSTEIN-JENSEN
BHI AGAR
BIRD SEED (NIGER) AGAR
THAYER-MARTIN (MTM) MEDIUM: This is used for the recovery of Neisseria spp.
MODIFIED THAYER-MARTIN MEDIUM: This medium is chocolate agar containing vancomycin, colistin, nystatin, and trimethoprim.
JEMBEC SYSTEM: This chocolate agar is a system for the recovery of Neisseria spp, requires CO2 for growth as the other chocolate agars do, and contains sodium bicarbonate and sodium citrate.
MODIFIED THAYER-MARTIN MEDIUM: This medium is chocolate agar containing vancomycin, colistin, nystatin, and trimethoprim.
JEMBEC SYSTEM: This chocolate agar is a system for the recovery of Neisseria spp, requires CO2 for growth as the other chocolate agars do, and contains sodium bicarbonate and sodium citrate.
TRIPLE SUGAR IRON AGAR (TSI)
UREASE AGAR
LIA AGAR
MOTILITY INDOLE ORNITHINE AGAR (MIO)
BHI BROTH (BRAIN-HEART INFUSION)
GN (GRAM-NEGATIVES) BROTH
LIM BROTH
QUAD PLATE
FASTIDIOUS ANAEROBE AGAR
CLOTEST rapid urease test agar to test for the presence of Helicobacter pylori.